SILAC refers to labeling cultured cells with heavy amino acids for quantitative proteomic tissue analysis. Labeling an entire proteome with heavy amino acids in vivo generates an ideal standard for quantitative proteomics. When a heavy labeled proteome is mixed with an unlabeled proteome then digested, every unlabeled peptide identified by the mass spectrometer can be quantified by its corresponding heavy peptide. In SILAC, the tryptic amino acids, arginine(R) and lysine (K), contain heavy stable isotopes, so if digesting with trypsin, every peptide is labeled. This metabolic labeling strategy has been employed by hundreds of proteomic studies.¹  The advantage of metabolic labeling over in vitro tagging techniques is that the heavy and unlabeled samples are mixed before sample preparation, preventing variability between preparations distorting the quantitation results.  This is especially important when extensive sample preparation (e.g. isolation of an organelle) is required.

CK Isotopes Ltd is the largest supplier of SILAC products in the UK and Ireland with next day delivery on many products and extremely competitive pricing for small and bulk orders.

All our SILAC products can be found in the literature below and please don’t hesitate to contact us for a quote or to discuss your requirements.

 

Application notes and FAQs

Below are the answers to some FAQs as well as a couple of application specific notes that may be of use.

  

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Metabolic Labeling (SILAC)

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